5-20 Thermo
Electron
January
2004
This opens the Select Wavelength Calibration dialog box shown in
Figure 5.14. There are 5 calibration files to choose from. The
holmuv.wcl uses 5 wavelengths in the UV region of the spectrum for
calibration. Files holm4.wcl, holm5.wcl and holm7.wcl use different
sets of wavelengths from both the UV and Vis regions. The
Custom.wcl file can be modified using a text editor program to create
a calibration file for a specific compound of interest.
Figure 5.14 Select Wavelength Calibration File
The expected holmium oxide absorbance maxima are selected from a
spectrum published in the ASTM Method E275-83 “Standard Practice
for Describing and Measuring Performance of Ultraviolet, Visible,
and Near Infrared Spectrophotometers.”
5.
Double-click on the desired file or highlight it and press the
Open button.
6.
The dialog box closes and the file name and its peaks are
displayed in the Wavelength File group as shown in
Figure 5.15.
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