sorin 75220, Operator'S Manual, Page: 201 / 304

Preoperative Sequestration (PPP and PRP)
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Operator’s Manual
20077/021 US
10-23
PRP1
The PRP1 protocol is intended to collect plasma, including platelet-rich plasma, into the PRP bag. The
remaining blood components will be emptied to the RBC bag. The PRP protocol is made up of three
phases: Fill, Spill, and Empty.
Fill Phase
The Fill phase of the PRP1 protocol is similar to that of the PPP protocol detailed in “PPP” on page
10-22, with exception that at the end of the Fill phase, while operating in Automatic mode, the
processing phase is automatically advanced to the Spill phase instead of to the Empty phase.
Spill Phase
Unlike the PPP protocol, the PRP protocols enter a Spill phase once the buffy coat is detected and the
PPP has been collected in the collection bag. The Spill phase makes possible further separation and
enrichment of the platelet harvest.
The first half of the Spill phase is the Spill PPP sub-phase. It consists of the following execution steps:
1. The pump stops and the centrifuge speed is steadily decreased to a set speed (2,400 RPM by
default). This slowing of the centrifuge de-compacts the red blood cells and enhances the buffy
coat.
2. The centrifuge spins for one minute at this slower speed with the pump stopped. This one-minute
delay serves to re-compact the buffy coat.
3. At the end of the one-minute delay, the pump resumes its rotation at the set speed until the set
PPP spill volume is transferred to the bowl (which causes an additional volume of PPP to spill into
the PRP collection bag).
The second half of the Spill phase is the Spill PRP sub-phase. It consists of the following execution
steps:
4. The pump stops for a second one-minute delay to again compact the buffy coat.
5. At the end of the one-minute delay, the pump resumes its rotation at the set speed until the set
PRP spill volume is transferred to the bowl (which causes an equal volume of PRP to spill into the
PRP collection bag).
6. After the set volume of PRP is spilled, the switch from the Spill phase to the Empty phase
happens automatically.
Empty Phase
The Empty phase of the PRP1 protocol, during which the remaining blood components in the bowl are
transferred to the RBC bag, is identical to that of the PPP and PRP2 protocols detailed in “PPP” on
page 10-22.
PRP2
The PRP2 protocol is intended to collect platelet-poor plasma in the PPP bag, and platelet-rich plasma
in the separate PRP bag. The remaining blood components will be emptied to the RBC bag. The PRP
protocol is made up of three phases: Fill, Spill, and Empty.
Fill Phase
The Fill phase of the PRP2 protocol is identical to the PRP1 Fill phase (refer to “PRP1” on page 10-23).
Spill Phase
The Spill phase of the PRP2 protocol is similar to the PRP1 Spill phase (refer to “PRP1” on page 10-23).
The difference is that between the Spill PPP and Spill PRP sub-phases, the operator will be asked to
clamp the PPP bag and open the PRP bag, allowing the PPP and PRP to be collected in separate bags.