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Preoperative Sequestration (PPP and PRP)
X
TRA
Operator’s Manual
20077/021 US
10-1
Chapter 10: Preoperative Sequestration (PPP and
PRP)
Description
The preoperative sequestration (SEQ) option of the
X
TRA
system allows separation of plasma and
platelets from whole blood previously collected in bags . Typically, whole blood separation is performed
so that the patient can receive a supply of autologous plasma containing clotting factors and platelets
at the end of a procedure. The
X
TRA
system allows for the collection of platelet-poor plasma (PPP) and
platelet-rich plasma (PRP) either in separate bags or together in the same bag.
The SEQ protocols (PPP, PRP1, or PRP2) are often referred to as sequestration and/or
preoperative protocols throughout this manual.
Familiarity with the intraoperative and postoperative operating procedures will be helpful in
understanding the preoperative sequestration procedures (refer to
Chapter 5: Processing ).
WARNING
Non-red cell components (e.g., platelet rich/platelet poor plasma
intended for transfusion and platelet rich/platelet poor plasma)
shall be used or applied before the patient leaves the operating
room or clinical procedure area.
i
i
American Association of Blood Banks. Standards for Perioperative Autologous Blood
Collection and Administration . 3rd Edition. Bethesda, MD. 2007 Reference Standard
5.1.8 (Handling, Storage, Transportation)
WARNING
Do not reinfuse the PRP back to the patient if the X
TRA
fails to
operate as intended.
WARNING
Whole blood must be anticoagulated as it is collected into bags
containing appropriate anticoagulant for plasma sequestration.
Inadequate anticoagulation may result in clotting, interfering with
the processing of the blood products.
WARNING
If plasma is being collected for transfusion, it must be transfused
before the patient leaves the operating room or clinical procedure
area.
i
i
American Association of Blood Banks. Standards for Blood Banks and Transfusion
Services. 18th ed. Bethesda, MD: American Association of Blood Banks, 1997.
WARNING
Do not overfill PPP and PRP bags. Overfilling may cause back
pressure that would cause fluid to exit through the bowl seal.
WARNING
To minimize the complications of particulate matter infusion and the
risk of air embolism, use of an in-line microaggregate filter on the
patient reinfusion line is STRONGLY RECOMMENDED.