sorin 75220, Operator'S Manual, Page: 200 / 304

Preoperative Sequestration (PPP and PRP)
10-22
20077/021 US
X
TRA
Operator’s Manual
PPP
The PPP protocol is intended to collect platelet-poor plasma in the PPP bag. The remaining blood
components will be emptied to the RBC bag. Unlike the other sequestration protocols, the PPP
protocol is made up of only two phases: Fill and Empty.
Fill Phase
The Fill phase of the PPP protocol is identical to that of the PRP protocols. It consists of the following
execution steps:
1. The centrifuge lid lock is engaged, the fill clamp is opened, and the centrifuge accelerates up to
the programmed speed (default is 5,600 RPM).
Note: If the bowl already contains blood and the buffy coat is detected before the centrifuge reaches
its programmed speed, the pump shall not start until 15 seconds after the centrifuge reaches its target
speed. This delay is intended to stabilize the buffy coat. The operator may manually start the pump
during this wait by touching the Play button. If the buffy coat is still detected after the delay, then the
pump is not started and the next step is skipped.
2. Once the centrifuge reaches its set speed, the pump begins to rotate counter-clockwise, pumping
blood from the whole blood bag to the bowl, at the factory defined speed (with a maximum
speed of 100 ml/min.). The pump continues to rotate at this speed until the bowl is 3/4 full of
concentrated red blood cells, at which point the Final Fill sub-phase begins.
3. During the Final Fill sub-phase, the pump speed is decreased in order to increase the thickness of
the buffy coat layer. (Modifications made by the operator to the pump speed during the Final Flow
sub-phase are reset to the factory settings at the next Fill phase.) The Final Fill sub-phase
continues until the buffy coat is detected, indicating that all the PPP has spilled into the PPP bag.
4. The next action after the buffy coat is detected depends on the operating mode:
◊ If Automatic mode is active, the switch from the Fill phase to the Empty phase happens
automatically.
◊ If Manual mode is active and the RBC detector is enabled, the system will issue a message
in the message area and stop the pump. The operator may then either continue with the
Fill phase or move forward to a different phase.
◊ If Manual mode is active and the RBC detector is disabled, no actions will be performed by
the system until the operator presses a key or some event is triggered (such as an alarm).
Empty Phase
The Empty phase of the PPP protocol is identical to that of the PRP protocols. It consists of the
following execution steps:
1. The centrifuge and pump stop, the fill clamp closes, and the empty clamp opens.
2. In order to move the remaining fluid from the bowl to the RBC bag, the pump begins rotating
clockwise with an appropriate acceleration. It reaches either the Empty factory pre-set pump
speed, or, if the pump speed has been modified by the operator in a previous cycle, the new
speed.
3. When air is detected in the line, the bowl is presumed empty and the pump stops, the empty
clamp closes, and the Ready screen is displayed on the touch screen.