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FluoroMax-3 v. 3.1 (3 Mar 2006)
Optimizing Data
5-4
Note:
Always remember that the focal point of the excitation beam
must be on the sample itself.
Ex
ci
ta
tio
n
Excitation
60°
Excitation monochromator
Emi
ss
ion
mo
no
ch
roma
to
r
Running a scan on a sample
Precautions with the Solid Sam-
ple Holder
Avoid placing the front face of the sample so that
the excitation beam is reflected directly into the
emission monochromator. If the sample is rotated
at 45° from excitation, this may occur, increasing
interference from stray light. Instead, set up the
sample with a 30° or 60°-angle to the excitation,
preventing the excitation beam from entering the
emission slits. The photograph at right illustrates
how a 60°-angle to the excitation keeps the
incoming excitation light away from the emission
monochromator’s entrance.
Use filters in the optical path.
Stray light from the excitation beam can interfere with the emission from the sample.
To reduce the deleterious effects of stray light, place a filter that removes excitation
wavelengths from the emission beam in the emission optical path. Here is an example
of scans with and without filters on a FluoroMax
®
-3, using an unknown white powder
as the sample. A
347 nm band-pass
filter allows only
the desired excita-
tion to reach the
sample, while a
long-pass filter in
the emission side
lets only fluores-
cence, and no stray
excitation into the
detector. Notice
how the shape of
the spectrum
changes drastically
when filters are
added.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...