FluoroMax-3 v. 3.1 (3 Mar 2006)
Troubleshooting
7-2
Raman band super-
imposed on fluores-
cence scan.
Aqueous solutions and
solvents have Raman
bands.
Change excitation wavelength to move Raman
band away from fluorescence peak, or run a
blank scan of the solvent and subtract it from
the fluorescence spectrum.
Large off-scale peak
at twice the excitation
wavelength.
Second-order effects from
the spectrometer.
Use cut-on filters to eliminate 2
nd
-order peak.
Scattered light off the exci-
tation wavelength.
Place bandpass filters in excitation light path.
Decrease emission-spectrometer slit widths.
Dirty cuvette.
Clean the cuvette as described in Chapter 5.
Stray light in emission
scan (also see example
in this chapter).
Solid-sample holder in
sample compartment.
Rotate the holder to prevent direct scatter from
entering the emission spectrometer.
Corrected excitation
spectrum curves up-
ward ~240–270 nm.
Dark count is divided by
low reference signal.
Use
Dark Offset
checkbox; retry scan.
Stirring speed is too fast.
Use slower stirring speed.
Noisy spectrum with
magnetic stirrer.
Stirring bar is too large;
light beam is striking it.
Use a smaller stirring bar (available from
BelArt Products, Pequannock, NJ).
Cables are improperly con-
nected.
Check communications cables’ connections.
BIOS is reset
Check the BIOS: See section “Checking the
FluoroMax
®
’s BIOS” later in this chapter.
Communication prob-
lems between com-
puter and instrument.
Computer’s or SpectrAcq’s
I/O-controller is failing.
Replace I/O controller: Call Spex
®
Fluores-
cence Service.
Hardware Init. error
appears.
Broken IR sensor in mono-
chromator.
Replace IR sensor: Call Spex
®
Fluorescence
Service.
Software is not enabled.
Check status.
Sample turret is not
operating.
Cables are connected im-
properly.
Check cable connections.
FluoroMax
®
-P resets
itself in phosphorime-
ter mode
Flash lamp’s life is expired,
drawing excess current,
causing transient emf inter-
ference.
Replace xenon flash lamp.
“Data file does not
exist” or “file read
error” message ap-
pears.
User is not logged into
Windows™ XP as admin-
istrator or power user.
Log into Windows™ XP as administrator and
or power user, and restart FluorEssence™.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...