FluoroMax-3 v. 3.1 (3 Mar 2006)
Optimizing Data
5-3
Dissolved solids
Solid samples, such as crystals, sometimes
are dissolved in a solvent and analyzed in
solution. Solvents, however, may contain
organic impurities that fluoresce and mask
the signal of interest. Therefore, use high-
quality, HPLC-grade solvents. If back-
ground fluorescence persists, recrystallize
the sample to eliminate organic impurities,
and then dissolve it in an appropriate solvent
for analysis.
Biological samples
For reproducible results, some samples may
require additional treatment. For example,
proteins, cell membranes, and cells in solution need constant stirring to prevent settling.
Other samples are temperature-sensitive and must be heated or cooled to ensure repro-
ducibility in emission signals.
Remove or change these metal spacers.
Solid
Sample
Holder
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...
