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FluoroMax-3 v. 3.1 (6 Mar 2006)
Glossary
13-11
easy control of assays and complex experiments.
Stokes shift
The energy difference between the absorption peak of lowest energy
and the fluorescence peak of maximum energy.
Synchronous scan
Scan type characterizing the overlap between the excitation and
emission. The excitation and emission spectrometers are scanned at
the same time, with a constant offset specified in either nanometers
(wavelength units) or in cm
–1
(energy units).
Technical spectrum
A spectrum acquired on research instrumentation with instrumental
bias remaining in the measurement. This spectrum must undergo
proper spectral correction in order to match the theoretical spectrum.
Spex
®
spectrofluorometers offer various methods for such correction,
including spectral correction, dark offset, blank subtraction, and oth-
ers.
Temperature scan
A FluorEssence™
Kinetics
scan-defintion that consists of a particu-
lar scan made across a user-defined temperature range. This scan
may be used to monitor a sample’s temperature response, or, more
specifically, to perform a melting curve for a sample. Temperature
scans require an automated bath compatible with FluorEssence™ to
be attached to the spectrofluorometer system along with a thermostat-
table sample mount.
Throughput
The amount of light that passes through the spectrofluorometer for a
particular measurement. The throughput usually is measured as the
counts per second measured on the water Raman band at 350-nm ex-
citation with 5-nm bandpass. As bandpass increases, so does the
throughput. Like bandpass, throughput has an inverse relationship
with resolution. When the throughput is increased, the resolution de-
creases.
Time-based scan
Scan type in which the sample signal is monitored as a function of
time, while both the excitation and the emission spectrometers re-
main at fixed wavelengths. Time-based data are used to monitor en-
zyme kinetics, dual-wavelength measurements, and determine reac-
tion-rate constants.
Total luminescence spec-
troscopy (TLS)
Spectroscopy devoted to monitoring changes to the entire excita-
tion/emission matrix of luminescence on a sample. This discipline is
best applied to fast kinetics measurements of samples during reac-
tions, temperature curves, or changes in other parameters.
Transmission
Light that passes through a sample without being absorbed, scattered,
or reflected. Transmission is usually measured as a percentage of the
incident light at a certain wavelength.
Triplet state (T
1
)
The spin-paired ground or excited state formed from the excited
singlet state, in which electrons are unpaired. The triplet state gives
rise to phosphorescence.
Tyndall scattering
Scatter that occurs from small particles in colloidal suspensions.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...