FluoroMax-3 v. 3.1 (7 Mar 2006)
Producing Correction Factors
8-1
8: Producing Correction Factors
Introduction
Gratings, detectors and other spectrometer components have response characteristics
that are functions of wavelength. These characteristics are superimposed on spectra,
and may yield a potentially misleading trace. For accurate intensity comparisons, such
as those required for quantum-yield determinations, spectrometer-response characteris-
tics must be eliminated. Corrections are made for each of these potential problems by
using radiometric correction factors.
Supplied with the FluoroMax
®
-3 are sets of excitation and emission correction factors
designed to eliminate response characteristics. These files,
xcorrect.spc
and
mcorrect.spc
, are included with the software and should be copied to the hard
disk. The excitation correction range is from 240–600 nm, and the correction range for
emission spectra is from 290–850 nm.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...
