FluoroMax-3 v. 3.1 (6 Mar 2006)
Glossary
13-4
monchromator to measure the fluorescence emission.
Emission scan
An acquisition that shows the spectral distribution of light emitted by
a sample. During an emission scan, the excitation spectrometer re-
mains at a fixed wavelength while the emission spectrometer scans a
user-selected region.
Energy transfer
The inter-molecular or intra-molecular transfer of the excited energy
from a donor to an acceptor. The transfer occurs without the appear-
ance of a photon and is primarily a result of dipole-dipole interac-
tions between the donor and acceptor.
Excitation/emission ma-
trix (EEM)
A three-dimensional plot showing the total luminescence from a
sample across all useful wavelengths. Total luminescence spectros-
copy is devoted to measurements of these EEMs for various materi-
als.
See also:
Multifile
,
Total Luminescence Spectroscopy
Excitation monochromator
The monochromator, located between the xenon lamp and the sample
compartment, used to isolate discrete wavelength components of the
excitation beam. This beam is directed to the sample, during which
the excitation monochromator may be used to scan the excitation
spectrum from a sample. The excitation monochromator on the
FluoroMax
®
-3 is an 0.18-m single monochromator with Czerny-
Turner design. This means that the monochromator includes a colli-
mating mirror, the reflection grating (blazed at 330 nm), and a focus-
ing mirror, with slit apertures at the entrance and exit. An excitation
shutter is located directly after the excitation exit slit to protect the
sample from photobleaching. The reference detector looks at a frac-
tion of the light exiting the excitation monochromator to correct for
the lamp response, if desired.
Excitation scan
A scan that reveals the spectral distribution of light absorbed by the
sample. The scan is collected by rotating the excitation grating while
holding the emission monochromator fixed. For non-ratiometric ac-
quisitions, acquire the scan using
S/R
to correct for the spectral out-
put of the lamp.
Excited state (S
1
)
The energy level to which an electron in the ground level of a mole-
cule is raised after the absorption of a photon of a particular wave-
length. Subsequently, fluorescence occurs, if the molecule returns to
the ground state via a radiative transfer from the S
1
state to the
ground state.
Experiment file
A file that contains specific information on the experimental setup for
an acquisition defined in
Experiment Setup
. This file is saved with a
default
*.EXP
extension. In addition to basic scan parameters, this
file saves system defaults (such as slit units), and some accessory set-
tings for the acquisition. Each acquisition type in the
Fluoresence
Experiment Menu
has its own default experiment file (e.g.,
DFLT0.EXP
is the default emission-scan definition). Use experi-
ment files to archive scan settings for acquisitions that are performed
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...
