FluoroMax-3 v. 3.1 (3 Mar 2006)
Optimizing Data
5-13
Smoothing data
Smoothing the data improves the appearance of the spectrum. Smoothing, as are most
post-processing features, is handled by Origin
®
.
1
Select Analysis
from the main
FluorEssence™
menu.
A drop-down menu appears.
2
Choose Smooth....
The
Smoothing
dialog box
opens.
3
Under the Settings tab, choose
the appropriate parameters.
4
Under the Operations tab,
choose FFT Filtering (fast-
Fourier transform), Adjacent
Averaging, or Savitsky-Golay
smoothing.
See the on-line Origin
®
help for additional information regarding smoothing data.
In general, start with a 9- or 11-point smooth for a time-base measurement. To select
the proper number of points for wavelength-scan types, first locate the area that re-
quires smoothing—usually this is a peak. Determine the number of data points used to
make up the peak, and then smooth the data using the number of points closest to this
number. To avoid artificially enhancing the data, use the appropriate number of points
to smooth the data. For example, selection of too large a number results in the back-
ground being smoothed into the peak.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...