horiba FluoroMax-3, Operation Manual

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FluoroMax-3 v. 3.1 (3 Mar 2006) Troubleshooting
7-8
Note:
If the instrument is out of calibration by more than 50 nm, the
Rayleigh scatter band (intense) can be mistaken at first glance for the
water Raman peak (much weaker). Try scanning to 600 nm (instead
of 450 nm) to help ensure that the water Raman peak is found.
If a spectrum similar to this is obtained after running a water Raman scan,
1
Rotate the cuvette 90° and rerun the scan.
If the problem goes away, then the problem was due to the cuvette surface. Clean or use
a different cuvette.
Or
1
Clean the cuvette.
2
Fill with fresh, double-distilled, deionized water.
If the problem goes away, then the problem was due to contaminated water.
Light not striking cuvette
The following graph shows a normal water-Raman scan with a superimposed problem
scan.
Here the problem is low intensity of the water signal when compared with the superim-
posed typical water Raman scan. To resolve this problem:
Low intensity during a Raman scan.
0
1
2
3
4
5
365 380 395 410 425 440 455
Wavelength (nm)
Intensity (10
5
counts/s)
Clean water
Low signal