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FluoroMax-3 v. 3.1 (6 Mar 2006)
Automated Polarizers
10-7
6
Apply the proper voltage on S (950 V for an
R928P photomultiplier).
7
Open the excitation shutter (if applicable).
8
Click the Accessories icon.
9
Set both polarizers to VV (0°).
10
Check the signal on S. Set slits evenly for each
monochromator to result in 1–1.5 million cps on
S.
11
Record the four permutations of the polarizers:
VV, HV, VH, and HH.
12
Subtract the dark counts from each permutation.
For example, VV
′
= VV – dark counts.
13
Calculate the polarization ratio using the dark-
subtracted data (e.g., VV
′
).
14
If the polarization ratio
≥
100, then the polarizers
are satisfactorily aligned. If not, then polarizers
should be re-aligned.
As an alternative, use an
Anisotropy
scan to acquire the polarization (
P
) or anisotropy
(<
r
>) to verify alignment. To be aligned,
P
≥
0.98 or <
r
>
≥
0.97.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...