horiba FluoroMax-3, Operation Manual, Page: 112 / 220

FluoroMax-3 v. 3.1 (7 Mar 2006) Producing Correction Factors
8-4
Note:
Naming the file
mcorrect
over-
writes the
mcor-
rect
file supplied
with the software.
a
Using the
Simple Math Tool
window
under the
Analysis
menu, divide
IRR
by
stdlamp2
, and name the
resulting file
mcorrect
.
mcorrect
IRR
stdlamp2
=
3
Normalize the new
mcorrect
file.
a
Display the new
mcorrect
file and find the minimum signal intensity.
To find the minimum quickly, right-click on the column of data’s header,
choose
Statistics on Columns
, and find the
Min(Y)
value.
b
Using the
Simple Math Tool
window under the
Analysis
menu, divide
the
mcorrect
file by this minimum signal intensity, a constant.
c
Save this new file as
mcorrect
.
(That is, overwrite the existing
mcorrect
file.) This normalizes the correction
factor file so that the minimum intensity of
mcorrect
will be 1 count s
–1
.
mcorrect
contains the emission correction factors for the system. The
correction-factor file should look similar to this:
mcorrect
file.
Once the emission correction factors have been found, determination of the excitation
correction factors may be necessary. The following procedures describe how to obtain
excitation correction factors using the photomultiplier and the photodiode. Follow the
procedure that applies to your configuration.