FluoroMax-3 v. 3.1 (6 Mar 2006)
FluoroMax
®
-P Phosphorimeter Operation
9-4
Initial Delay
can be varied with time to yield a decay curve. Spec-
tra can be scanned to isolate different phosphorescing components
based on the lifetime of the luminescent species in the sample. To-
gether, these two techniques can be used to create three-
dimensional plots. For example, successive scans with varying de-
lay times can be plotted.
To record fluorescence and phosphorescence
emission, set
Initial
Delay
to zero.
Sample
Win-
dow
Sets the duration of signal acquisition, in ms. The
Sample Win-
dow
opens when the
Initial Delay
ends. When the
Sample Win-
dow
opens, the signal is counted and integrated. After the
Sample
Window
closes, any signal is ignored.
The
Sample Window
may be set from 0.01 to 10 000 ms. If the
lifetime of the phosphorescence is known, set the
Sample Window
to 5–10 times the lifetime. If the phosphorescence lifetime is un-
known, make the
Sample Window
a small fraction of the antici-
pated lifetime, and then increase it until acceptable results are ob-
served.
If the
Sample Window
is too long, the detector will record spuri-
ous background signal. If the
Sample Window
is too short, com-
ponents of the lifetime decay may be missed. With two or more
species decaying simultaneously, try varying the
Initial Delay
and
the
Sample Window
.
Time per
flash
Sets the total cycle length per flash, including on time, decay time,
and dead time between flashes. The
Time per flash
is the recipro-
cal of the repetition rate of the lamp pulses. The allowable repeti-
tion rate is 0.03–25 Hz. The
Time per flash
must be slow enough
to let the
Sample Window
close before another flash begins. Ac-
curacy of the repetition rate is ± 1 ms. The
Time per flash
is gov-
erned by
Time per Flash
≥
Initial Delay
+
Sample Window
+ 20 ms
Flash count
Sets the number of lamp pulses that contribute to each data point.
The range for
Flash count
is 1 to 999. The signal collected per
flash is integrated over the total
Flash count
s before FluorEs-
sence™ stores it. The more flashes accumulated, the higher the sig-
nal-to-noise ratio becomes.
For more information about FluorEssence™ phosphorimeter commands, consult the
FluorEssence™ on-line help.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...
