FluoroMax-3 v. 3.1 (6 Mar 2006)
Automated Polarizers
10-21
Problem Cause
Possible
Remedy
Improper sample concen-
tration
Adjust sample concentration.
Photomultiplier saturated;
slits improperly set
Check that sample signals are
in linear region (< 2 × 10
6
cps
on S or T, < 10 µA on R). Re-
set slits.
Dirty cuvette
Clean the cuvette.
Polarizer misaligned
Check polarizer alignment.
Poor polarization data
System misaligned
Check system alignment in a
generic layout. Run lamp scan
and water Raman scan to
check calibration.
Highly concentrated stan-
dard
Check Ludox
®
or glycogen
concentration: higher concen-
trations can cause inner-filter
effect, lowering ratio.
Improperly set slits
Set slits for ~ 1 × 10
6
cps in
VV. Signals much less than
this give excessive contribu-
tion from dark noise, while
signals > 2 × 10
6
cps are in
non-linear region.
Low polarization ratio
System misaligned
Check system alignment in
generic layout. Run lamp scan
and water Raman scan to
check calibration.
Wrong instrument configu-
ration is loaded
Check that a configuration
with autopolarizers is loaded.
Autopolarizers do not
initialize (they do not
move during initializa-
tion).
Bad cable connections
With the system power off,
recheck cable connections.
Wrong instrument configu-
ration is loaded
Check that a configuration
with autopolarizers is loaded.
Bad cable connections
With the system’s power off,
recheck cable connections.
Software failure initial-
izing autopolarizers
Computer hang-up
Exit the software, and reboot
the system and host computer.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...