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FluoroMax-3 v. 3.1 (3 Mar 2006)
Optimizing Data
5-1
Note:
Clean the sample
cells thoroughly before
use to minimize back-
ground contributions.
Warning:
Nitric acid is a dangerous substance.
When using nitric acid, wear safety goggles,
face shield, and acid-resistant gloves. Certain
compounds, such as glycerol, can form explo-
sive materials when mixed with nitric acid. Refer
to the Materials Safety Data Sheet (MSDS) for
detailed information on nitric acid.
Caution:
Soaking the cuvettes for a long period
causes etching of the cuvette surface, which re-
sults in light-scattering from the cuvettes.
5: Optimizing Data
Spectra can be enhanced by optimization of data-acquisition. This chapter lists some
methods of optimizing sample preparation, spectrofluorometer setup, and data correc-
tion to get higher-quality data.
Cuvette preparation
1
Empty all contents from
the cuvettes.
2
Fully immerse and soak
the cuvettes for 24 h
in 50% aqueous ni-
tric acid.
This cleans the cuvettes’ inner and
outer surfaces.
3
Rinse
with de-ionized water.
4
Clean the cuvettes in the cleaning solution with
a test-tube brush.
Use Alconox
®
or equivalent detergent as a cleaning solution.
5
Rinse the cuvettes with de-ionized water.
6
Soak the cuvettes in concentrated nitric acid
.
7
Rinse them with de-ionized water before use.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...