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FluoroMax-3 v. 3 (22 Feb 2005)
System Description
2-3
Left: motor and gear-
box that rotate the mir-
ror to switch lamps.
Right: Twin sources in
the FluoroMax
®
-P.
CW lamp
flash lamp
In the FluoroMax
®
-P, a second source, a xenon flash lamp, is available also. A FluorEs-
sence™-controlled motor and gearbox rotate the mirror in the illumination area, in or-
der to switch between the CW source and the flash lamp.
Monochromators (2 and 4)
The FluoroMax
®
-3 contains Czerny-Turner monochromators for excitation and emis-
sion. The Czerny-Turner design uses all-reflective optics to maintain high resolution
over the entire spectral range, and minimize spherical aberrations and re-diffraction.
Gratings
The essential part of a monochromator is a reflection grating. A
grating disperses the incident light by means of its vertical grooves.
A spectrum is obtained by rotating the gratings, and recording the
intensity values at each wavelength. The gratings in the Fluoro-
Max
®
-3 contain 1200 grooves mm
–1
, and are blazed at 330 nm
(excitation) and 500 nm (emission).
Blazing
is etching the grooves
at a particular angle, to optimize the grating’s reflectivity in a par-
ticular spectral region. The wavelengths selected are optimal for
excitation in the UV and visible, and for emission in the high-UV to
near-IR. Each grating is coated with MgF
2
for protection against oxidation. The system
uses a direct drive for each grating, to scan the spectrum at up to 200 nm s
–1
, with accu-
racy better than 0.5 nm, and repeatability of 0.3 nm.
Slits
The entrance and exit ports of each monochromator have continuously adjustable slits
controlled by FluorEssence™. The width of the slits on the excitation monochromator
determines the bandpass of light incident on the sample. The emission monochroma-
Excitation monochromator.
Emission
monochromator.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...