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FluoroMax-3 v. 3.1 (7 Mar 2006)
Producing Correction Factors
8-7
The largest lamp peak occurs at 467 nm.
d
Set the slit on the excitation monochromator to 0.5 mm.
e
Make sure the shutter is open.
f
Adjust the slit on the emission monochromator (
Slits Width
field) to
obtain a signal intensity of (1 ± 0.1) × 10
6
cps.
Note the slit-width: the slit-width discovered in this step will be used to run the
scan.
g
Close the
Real Time Control
and revert to the
Experiment Setup
dialog
box.
h
Close the dialog box and open the
Experiment Setup
dialog box for an
excitation scan instead.
4
Set up the excitation-correction scan.
a
Enter the scan parameters:
Excitation Wavelength Start (nm)
240
Excitation Wavelength End (nm)
600
Inc (nm)
5
Emission Wavelength Park
650
Excitation Slit (nm)
0.5
Emission Slit (nm)
Use value determined in step
3f
.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...