horiba FluoroMax-3, Operation Manual

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FluoroMax-3 v. 3.1 (3 Mar 2006)
iii
Table of Contents
0: Introduction ................................................................................................ 0-1
About the FluoroMax
®
-3 and FluoroMax
®
-P........................................................................................... 0-1
Chapter overview.................................................................................................................................... 0-2
Disclaimer ............................................................................................................................................... 0-3
Safety summary...................................................................................................................................... 0-5
Risks of ultraviolet exposure................................................................................................................... 0-7
Additional risks of xenon lamps ............................................................................................................ 0-10
1: Requirements & Installation ............................................................................ 1-1
Safety-training requirements .................................................................................................................. 1-1
Surface requirements ............................................................................................................................. 1-2
Environmental requirements................................................................................................................... 1-3
Electrical requirements ........................................................................................................................... 1-4
Unpacking and Installation ..................................................................................................................... 1-5
Software emulation ................................................................................................................................. 1-9
2: System Description....................................................................................... 2-1
Introduction ............................................................................................................................................. 2-1
Basic theory of operation........................................................................................................................ 2-1
Optical layout .......................................................................................................................................... 2-2
3: System Operation ......................................................................................... 3-1
Introduction ............................................................................................................................................. 3-1
Controls and indicators........................................................................................................................... 3-1
Turning on the system ............................................................................................................................ 3-2
Checking system performance ............................................................................................................... 3-3
4: Data Acquisition........................................................................................... 4-1
Experiment Menu button ........................................................................................................................ 4-2
Previous Experiment Setup button ......................................................................................................... 4-4
Auto Run Previous Experiment button ................................................................................................... 4-5
Make Overlay File button........................................................................................................................ 4-6
3D Scan to 3D Profile button .................................................................................................................. 4-7
Get Peak Information button................................................................................................................... 4-9
Running an unknown sample ............................................................................................................... 4-10
5: Optimizing Data ........................................................................................... 5-1
Cuvette preparation ................................................................................................................................ 5-1
Sample preparation ................................................................................................................................ 5-2
Running a scan on a sample .................................................................................................................. 5-4
Measuring the
G factor ........................................................................................................................... 5-6
Improving the signal-to-noise ratio ......................................................................................................... 5-8
Correcting data ..................................................................................................................................... 5-14
6: Maintenance............................................................................................... 6-1
Introduction ............................................................................................................................................. 6-1
Lamp replacement .................................................................................................................................. 6-1
Electronics .............................................................................................................................................. 6-9
7: Troubleshooting........................................................................................... 7-1
Chart ....................................................................................................................................................... 7-1
Checking the FluoroMax
®
’s BIOS........................................................................................................... 7-3
Using diagnostic spectra ........................................................................................................................ 7-5
Further assistance… ............................................................................................................................ 7-10