horiba FluoroMax-3, Operation Manual, Page: 146 / 220

FluoroMax-3 v. 3.1 (6 Mar 2006) Automated Polarizers
10-12
10
Open the excitation shutter (if applicable).
11
Turn on high voltage and set appropriately for S
channel (950 V for R928P; 1050 V for R1527).
12
Set slits to 5-nm bandpass for all monochroma-
tors.
13
Set scatterer concentration to give 1–1.5 × 10
6
cps on S.
14
Rotate the excitation polarizer to a rough maxi-
mum.
15
Set the polarizers to HV (90°, 0°) and rotate the
excitation polarizer for the minimum signal on S.
16
Set the polarizers to VH (0°, 90°) and rotate the
emission polarizer for the minimum signal on S.