FluoroMax-3 v. 3.1 (7 Mar 2006)
Producing Correction Factors
8-3
Calculating emission correction factors
Introduction
For more information about the theory and application of radiometric correction, con-
sult
Accuracy in Spectrophotometry and Luminescence Measurements
, Mavrodineau,
Schultz, and Menis, NBS Spec. Publ. 378 (1973), especially p. 137, “Absolute Spectro-
fluorometry,” by W.H. Melhuish.
Irradiance values for a standard lamp, packaged with the lamp, usually are expressed in
10
–6
W·cm
–2
·nm. With photon-counting systems like the FluoroMax
®
-3 spectrofluoro-
meter, however, data usually are collected in units of photons·s
–1
·cm
–2
·nm. To convert
the units, multiply each irradiance value by the wavelength at which it is valid. (The
data will still be off by a factor of
c
, but normalizing the correction factors compensates
for this.) Such a mathematical procedure can be done in a spreadsheet program.
1
Load the irradiance values.
a
Enter the irradiance values into a spreadsheet.
Use the Origin
®
project supplied by HORIBA Jobin Yvon.
b
Save the file as
IRR
.
Now you have the two files:
IRR
and
stdlamp2
. These files are required to
calculate the emission correction factors for the FluoroMax
®
-3 system.
2
Calculate the correction factors.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...
