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FluoroMax-3 v. 3.1 (3 Mar 2006)
Optimizing Data
5-8
Improving the signal-to-noise ratio
Because of various hardware or software conditions, occasionally it is necessary to op-
timize the results of an experiment.
The quality of acquired data is determined largely by the signal-to-noise ratio (
S
/
N
).
This is true especially for weakly fluorescing samples with low quantum yields. The
signal-to-noise ratio can be improved by:
•
Using the appropriate integration time,
•
Scanning a region several times and averaging the results,
•
Changing the bandpass by adjusting the slit widths, and
•
Mathematically smoothing the data.
The sections that follow discuss the alternatives for improving the
S
/
N
ratio and the ad-
vantages and disadvantages of each.
Summary of Contents for FluoroMax-3
Page 5: ...FluoroMax 3 v 3 1 3 Mar 2006 v 15 Declaration of Conformity 15 1 16 Index 16 1...
Page 6: ...FluoroMax 3 v 3 1 3 Mar 2006 vi...
Page 18: ...FluoroMax 3 v 3 1 3 Mar 2006 Introduction 0 12...
Page 36: ...FluoroMax 3 v 3 22 Feb 2005 System Description 2 8...
Page 96: ...FluoroMax 3 v 3 1 3 Mar 2006 Maintenance 6 12...
Page 156: ...FluoroMax 3 v 3 1 6 Mar 2006 Automated Polarizers 10 22...
Page 208: ...FluoroMax 3 v 3 1 6 Mar 2006 Bibliography 14 4...
Page 210: ...FluoroMax 3 v 3 1 6 Mar 2006 Declaration of Conformity 15 2...