horiba FluoroMax-3, Operation Manual, Page: 110 / 220

FluoroMax-3 v. 3.1 (7 Mar 2006) Producing Correction Factors
8-2
Generating emission correction factors
Generate a new correction-factor file only when the gratings or detectors have been re-
placed with those of different specifications than the original hardware.
Required kits
Emission correction factors should be updated periodically or whenever different grat-
ings or signal detectors are installed. The correction factors can be updated either at the
user’s location, or by a representative from the Spex
®
Fluorescence Service Depart-
ment. To arrange for a visit and a fee estimate, call Spex
®
Fluorescence Service De-
partment. To update the correction factors without a service visit, call Spex
®
Fluores-
cence Service Department for instructions and further information.
One way to generate correction factors for the instrument is to scan the spectrum of a
standard lamp. Because the actual irradiance values of the standard lamp as a function
of wavelength are known, dividing the irradiance values by the lamp spectrum results
in a set of relative correction values. These values can then be applied to the raw fluo-
rescence data. The emission correction factor file
mcorrect.spc
was acquired in
this manner.
To generate emission correction factors, several items are needed: a standard lamp, ap-
propriate holders, and a scatter assembly. HORIBA Jobin Yvon Inc. offers two kits: the
Model 1908 Standard Lamp Accessory, and the Model 1908MOD Scatter Assembly.
The Model 1908 is a complete correction factor kit, while the Model 1908MOD Scatter
Assembly is provided for users who already have a calibrated standard lamp and a con-
stant-current source.
The Model 1908 Standard Lamp Assembly is a complete correction factor kit, which
includes the following items:
•
200-watt quartz tungsten-halogen filament lamp with irradiance values
•
Constant-current power supply with lamp holder
•
1908MOD scatter assembly
The Model 1908MOD scatter assembly includes:
•
Lamp-mount assembly and mask with square center
•
Scatter block with neutral-density filter and reflectance plate