Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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6. Configure the remaining options.
Option Description
Initial Well for
Finding
Sample Sets the well for the first Find Sample autofocus. Select one of the following:
First Well Acquired: Finds the sample autofocus using the first well that is
acquired. Recommended setting for most protocols.
Specific Well: Finds the sample autofocus using a well that you specify.
Note: After you select this option, you must specify the well row and
column number. A1 is the default value.
Skip Find Sample: Select if sample is already in focus. Disables the initial
Find Sample autofocus when starting to acquire a plate. Select this option
when the sample is already in focus.
Note: Select this option when you use an oil-immersion objective,
and you must manually find the focus before starting acquisition.
Number of
Wells to
Attempt
Initial Find
Sample Enabled only if you select the First Well Acquired option. The first well in which
a sample is found is the well the software uses for autofocusing. The default
value is one.
Note: If you use a robot for automated plate loading, set this value to
three or greater.
Site
Autofocus Enabled only if you select Fixed Number of Sites or Adaptive Acquisition on the
Sites to Visit tab. This section includes several options to configure site-to-site
autofocus. Select one of the following:
First Site Only: Autofocuses in the top-left site in the well.
Center of Well Only: Autofocuses in the center of the well.
Note: Select this option when the magnification is low and the sites
are relatively close together; also select this option for round-
bottom (U-bottom) plates.
All Sites: Autofocuses for each site.
Note: Select this option with higher magnification, when the sites
are spread far apart, or there is extreme variation in the plate bottom.
Timelapse
Autofocus Enabled if you select Acquire Time Series on the Acquisition tab. See
Configuring Series Acquisition Options on page 106 . Select one of the following:
First Timepoint Only: Use for fast kinetic acquisitions.
All Timepoints: Use for long time lapse acquisitions.
Every N
th
Timepoint: Set a value that is less than the number of time points.
See Configuring Continuous Time Series Acquisitions on page 107 .
View
Focusing
Details Displays the Auto Focus Details dialog that displays the current autofocus
parameters. This information is useful for diagnostic purposes when
troubleshooting focusing issues.
Click Copy to copy the parameters to the clipboard and then paste them into a
third-party application, such as Microsoft Word.
Table 5-6: Autofocus Tab, General Options