Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Option Description
Before Each
Image Runs during the acquisition loop, after the illumination is set and focusing is
complete.
After Each
Image Runs during the acquisition loop, after the shutter closes and before images
save.
Before
Focusing Runs during the acquisition loop, just before the focus step begins.
Start of Z Runs before a Z series is acquired. The journal runs when the Z motor is at the
lowest step in the series and before an image is acquired. The journal runs
once for each wavelength that you configure to use the Z series.
End of Z Runs after a Z series acquisition completes. The journal runs when the Z
motor is at the highest step in the series and after any projection images are
generated. The journal runs once for each wavelength that you configure to
use the Z series.
Start of Site Runs during the acquisition loop, before any images are acquired from each
site.
End of Site Runs during the acquisition loop, after all images have been acquired from
each site.
Start of Well Runs during the acquisition loop, at the beginning of each well, before any
images are acquired from a well.
End of Well Runs during the acquisition loop, at the end of each well, after all images have
been acquired from a well.
Start of Time
Point Runs during the acquisition loop, at the beginning of each time point, before
any images are acquired for each time point.
End of Time
Point Runs during the acquisition loop, at the end of each time point, after all images
have been acquired for each time point.
Start of Plate Runs before the initial find sample step is performed. Cannot be used to
modify the Find Sample location.
End of Plate Runs after the last image acquisition for a plate is complete.
Prevent
Asynchronous
Hardware
Moves (Optional) Select this option only if a journal moves hardware, such as
changing filters, or moving focus.
This option ensures that the journals run correctly.
Note: Do not select this option without the use of a journal because it can
slow the acquisition.
Table 5-13: Journal Acquisition Steps