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Chapter 7: Expansion Solution Options
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Performing Time Lapse Experiments
The ImageXpress Environmental Control option incubates live cells, enabling imaging
experiments to be performed over hours or days. Wells can be imaged repeatedly at fixed
time intervals, and movies can be constructed from a series of images.
Note:
In time lapse acquisitions, the time starts when the first well is acquired. If the
acquisition time exceeds the time specified for the time lapse experiment, the
system proceeds as fast as possible. As a result, the time between time points
might not match the time specified, but timestamps on images are accurate.
In time lapse acquisitions, the image save option for the Z option is to save the 2D
projection images.
To set up a time lapse experiment:
1. In the MetaXpress software, do one of the following to display the Plate Acquisition Setup
dialog:
From the simplified menu, click Screening > Acquisition Setup.
From the standard menu, click Screening > Plate Acquisition Setup.
2. On the Configure tab, select the Acquisition tab.
Figure 7-22: Plate Acquisition Setup Dialog, Acquisition Tab
3. Select the Acquire Time Series check box to add a Timelapse tab to the dialog.