Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

Страница: 148 / 256

ImageXpress Confocal HT.ai High-Content Imaging System User Guide
148 5084773 A
Loading the Sample Plate
You must use the live-cell sealing ring to maintain the CO
2
flow to the sample plate. The
sealing ring helps to contain the inputted air directly over the cells and maintains the proper
CO
2
and temperature levels.
WARNING! BIOHAZARD.
Wear gloves when handling sample plates.
Note: To ensure that it is at the proper temperature, before you load the sample plate,
make sure that the live-cell sealing ring is in the system or in the incubator.
To load the sample plate:
1. In the MetaXpress software, do one of the following to open the instrument top door:
From the simplified menu, click Screening > Acquisition Setup > Eject Plate.
From the standard menu, click Screening > Plate Acquisition Setup > Eject Plate.
2. Insert the sample plate into the stage and then remove the lid from the plate. If there are
concerns about contamination, a breathable seal can be used over the top of the plate.
3. Place the live-cell sealing ring directly on top of the plate, making sure that it fits securely
onto the plate.
Figure 7-20: Environment Control Live-cell Sealing Ring
4. In the MetaXpress software, do one of the following to close the instrument top door:
From the simplified menu, click Screening > Acquisition Setup > Load Plate.
From the standard menu, click Screening > Plate Acquisition Setup > Load Plate.
Note: Temperature fluctuations in the plate and its surroundings
cause the plate and its cells to shift in X, Y, and Z direction. To
minimize these temperature fluctuations, take the following
precautions.