Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

Страница: 222 / 256

ImageXpress Confocal HT.ai High-Content Imaging System User Guide
222 5084773 A
Determining the Plate Bottom Reference Point After Changing the Reference
Objective
Note: Do this procedure only if you replace the reference objective. If you did not
replace the reference objective, see
Entering the Focus Objective Values in the Meta
Imaging Series Administrator Software on page 228 .
The plate bottom reference point is a setting that the MetaXpress software uses for auto focus.
Plate bottom reference is configured before the system is shipped. The reference point is
determined using a particular objective (usually 10x) in a specific objective position. If you
change or move this objective, you must determine the new plate bottom reference point and
enter this value in the Meta Imaging Series Administrator Software.
Note: Whenever possible, leave the reference objective in place and replace the other
objectives.
There are two separate procedures to update the plate bottom reference point:
Finding the Plate Bottom Reference Objective and Reference Point on page 223
Entering the Plate Bottom Reference Objective Value in the MetaXpress.ref Configuration
File on page 227
Note: You should contact technical support before you attempt these procedures. See
Obtaining Support on page 237 .
The objective position used for this setup is specified in a line of the system calibration file
MetaXpress.ref, located in the Hardware folder of the root install directory (C:\MX6\Hardware
by default).
The line is:
[system calibration]
PlateBottomReferenceObjective= X
Where X is the position of the objective used as the plate bottom reference objective
(between 1 to 4).
If you update the plate bottom reference point, you must update the value in the calibration
file.