Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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4. Set up other illumination settings if needed.
The value in the Wavelength field should match the center wavelength for the emission
filter.
CAUTION!
Never set more than one 89 North shutter to Active.
Never set an 89 North shutter to Open.
5. In the Name field, enter the name of the new filter cube.
6. In the Wavelength field, enter the center emission wavelength of the new filter cube.
7. Click Add/Replace to add the setting to the Defined Settings list.
8. If you replace a filter that had an existing setting, select the old setting in the Defined
Settings list and click Remove.
9. Click Backup to back up the settings to a file.
10. Click Close.
Settings for Common Fluorophores
Fluorophore Shutter Laser Line Excitation Filter
DAPI Violet (405 nm) 405/20 nm
CFP Blue (445 nm) 445/20 nm
FITC Cyan (467.5 nm) 467.5/21 nm
YFP Teal (520 nm) 520/10 nm
TRITC and Texas Red Green (555 nm) 555 nm
Cy5 Red (638 nm) 638/17 nm
Cy7 Near Infrared (725 nm) 725/30 nm
Table 8-1: Settings for Common Fluorophores