Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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Chapter 1: ImageXpress Confocal HT.ai System
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Objective Lenses
The ImageXpress Confocal HT.ai system can be configured with high-quality Nikon objectives.
See
Compatible Objectives on page 243 .
If the objective you want to use is not listed, contact Molecular Devices to verify compatibility
with your system.
Note: Some objectives have adjustable spherical aberration correction collars for
imaging through thick substrates such as most microplates. Use the software to
calculate and set the correct values. See
Adjusting Objective Correction Collars on
page 213 .
Several objectives (for example, the 40x Super Plan Fluor ELWD) have correction collars for
adjustment to the thickness of the glass cover slip or plate bottom you use. Adjust the collars
using the physical thickness of the plate bottom or through optimization of image quality.
Objectives are classified according to optical correction, flatness of field, numerical aperture,
and working distance. Before you choose the objectives to use with the system, it is important
to consider the types of plates and type of assay you plan to image. The plate material (plastic
or glass) and thickness are major considerations when you choose an objective. Another
important note is that generally the more optically corrected an objective, the greater the
number of lens elements it contains, with correspondingly reduced light transmission,
especially in the UV spectrum. The levels of correction in descending order are as follows:
Achromatic
Fluor
Plan Fluor
Plan Apo
CAUTION! You must contact Molecular Devices Technical Support to schedule a Field
Service Engineer to install or remove a water immersion objective.
For detailed information on objectives, see the Nikon web site ( www.nikon.com )