Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Option Description
Magnification Selects the magnification setting to assign X and Y offset values (parcentricity)
and a Z offset (parfocality) to a specific objective. When you select an objective,
the objective physically moves to the acquisition position.
Note: Define the magnifications in the Configure Magnification dialog. See
Verifying Magnification Settings on page 42 . You must assign a calibration
to each magnification setting. See Verifying Calibration Settings on page
44 .
Camera Binning Specifies the binning value to apply to the camera. Binning combines the output
of adjacent pixels in square multiples. For example, a camera binning value of 1 is
one pixel, a binning value of 2 combines 2x2 or four pixels in a square, a binning
value of 3 combines 3x3 or nine pixels in a square, and so on. This reduces the
image file size and resolution and increases signal-to-noise ratio.
Note: If sufficient light is available, lower camera binning increases the
image resolution, whereas higher binning increases the signal-to-noise
ratio for a given exposure time. Higher binning also improves the speed
of the acquisition for a given target signal per bin.
Acquisition
Mode Specifies widefield or confocal imaging mode options, which are dependent on
the instrument configuration.
Note:
An entire acquisition is either widefield or confocal. You cannot
change the acquisition mode from time point to time point or from
wavelength to wavelength.
Changing the Acquisition Mode automatically adjusts the
Illumination Power settings for all wavelengths. You may need to
reconfigure these settings for the new acquisition mode.
Table 5-1: Configure Tab, Objective and Camera Tab Configuration Options