Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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Chapter 4: Preparing for Acquisition
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Z Series Acquisition
The nature of your sample material can be very dense or thin. Dense sample material requires
more light and might require collection of multiple focal planes in a Z Series. Depending on the
analysis needs, you can save individual planes or collapse planes into a single 2D projection
image.
In Transmitted Light, collecting z planes of cells growing throughout a 3D gel matrix cannot
give an accurate image of all planes because the transmitted light must pass completely
through the sample from top to bottom so that objects high in the sample will block the light to
planes below it.
Instrument Maintenance
For best performance, your ImageXpress Confocal HT.ai system should have regular
preventive maintenance services. In between preventative maintenance services, your
system administrator can clean dust off the optics as needed. Wear gloves when you handle
any optical components to avoid contaminating them with dirt or skin oils. See
Maintenance on
page 189 .
The basic design of the ImageXpress Confocal HT.ai system light source and light path within
the instrument help ensure that the light that reaches the sample is the best possible quality. If
you find that the light quality has become degraded, contact your system administrator or your
Molecular Devices representative to correct the problem.