Molecular Devices ImageXpress Confocal HT.ai, Руководство пользователя

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Drag the Current Position flag on the Z series diagram to update the current position
for the Z motor. After you move the flag, you can then click Set T or Set B to set the
top and bottom positions.
Note: If the Current Position flag does not display, use the Focus active
wavelength tool first.
Tip:
Use this function with the Start Live active wavelength tool. Click Start Live,
and then with the Focus set, click and drag the Current Position flag to view
the different positions in an image in real time. Use this approach to
interactively visualize the location for the top of the cells in the sample. You
could then click Set T on the Current Position flag to accurately set the
position for the top of an image.
Live mode is more responsive when binning is set to 2 or higher.
Click on a blue bar in the Z series diagram to move the Current Position flag to this
plane. (This flag indicates the current position for the Z motor.) When you are in Live
mode, when you click on different planes in the Z series diagram, the image in the
Snap window updates accordingly.
Tip:
In Test mode, when you capture a Z stack, an image window displays. Click
and drag the slider bar at the top of the dialog to scroll through the Z stack
images.
In Start Live mode, only the current plane displays. To change the current
plane view, click on the step markers in the ruler on the Z tab.
To move the Z motor up or down one step at a time by the recommended Step Size,
click the small step arrows and . To move the Z motor up or down one step at a
time by the defined Step Size, click the large step size arrows to the right of the Z
series diagram. Click and hold a step size arrow to continuously move the Z motor.
4. When you finish configuring the protocol, continue to Saving Plate Acquisition Protocols
on page 124 .