Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Taking a Sample from the Sample Port
1. Open the incubator door.
2. Use a cleansing wipe that contains an appropriate laboratory disinfectant to clean the sample port
surface that will interface with the syringe.
3. Connect the syringe to the sample port, as shown in Figure 5-6.
Figure 5-6: Sample port with the syringe connected
Note: Performing step 4 removes any residual media from the filter, which ensures that you are testing media
that is currently circulating in the EC circulation loop.
4. Draw 3 mL of media through the filter and the sample port, and then discard the media.
5. Repeat step 2 to clean the sample port.
Note: To avoid drawing waste into the EC circulation loop, draw the minimum volume from the sample port
that is appropriate for the desired analysis, such as measuring the glucose or lactate levels. This sampling
method is not appropriate for measuring gas concentrations.
6. Draw the volume of sample needed.
7. Repeat step 2 to clean the sample port.
8. Close the incubator door.
Taking a Sample from the Sample Port
Quantum
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Cell Expansion System • Operator's Manual for Software Version 2.1 5-7