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7-Step Quick Reference Guide

3M

TM

 Attest

TM

 390 Auto-reader and 3M

TM

 Attest

TM

 Rapid Readout Biological Indicator (BI)

Refer to 3M

TM

 Attest

TM

 Rapid Readout Biological Indicator (BI) package inserts for complete instructions. 

Refer to 3M

TM

 Attest

TM

 390 Auto-reader Operator’s Manual for complete instructions.

3M

TM

 Attest

TM 

Rapid 

Readout Biological 

Indicator (BI)

3M

TM

 Attest

TM 

390 

Auto-reader

 

Ŕ Allow BI to cool at least 10 minutes.

 

Ŕ Crushing or excessive handling of the BI before cooling may cause 

the glass ampoule to burst.

 

Ŕ While holding BI in hand, press the cap down.

 

Ŕ Hold BI by the cap and use “Crusher Wall” to crush the BI only one time.

 

Ŕ

DO NOT

 use your fingers to crush the glass ampoule.

 

Ŕ Hold the BI by the cap and tap bottom of vial on countertop until 

the media wets spore strip at bottom of vial.

 

Ŕ

DO NOT

 roll BI between fingers.

 

Ŕ Place BI into incubation well that corresponds with colour-coded 

BI cap. Observe LCD panel to confirm BI is correctly seated. 

Remaining incubation time, in minutes, will be displayed directly 

below BI on the LCD display to indicate incubation is in progress. 

 

Ŕ

DO NOT REMOVE BI

 from the incubation well until the 

(+) or (-) symbol on the LCD panel indicates the test is complete.

 

Ŕ Record result for both test BI (processed) and positive control (unprocessed).

 

Ŕ Record result for both test BI (processed) and positive control (unprocessed).

NOTE

: Do not reincubate the same BI for a second reading as any subsequent readings would be considered invalid.

Unprocessed Controlled 

Incubated

Processed 

Incubated

Processed

Cool

Close

Crush

Tap

Incubate

Record

Interpret 

Results

1

2

3

4

5

7

6

File Name : 1708-09713 _Revise390_6StepGuide.indd

File Description : Attest Quick Step 1 sheet  (Cncpt #2 Front) 

File Size : 11” W x 17” H plus bleed and trim 

Colour : 4 Colour Process

ATTENTION:

 Wear safety glasses and gloves. 

DO NOT

 place another label or indicator tape on the vial or on the cap of the BI.

Ŕ

Ŕ

Available in Canada from:

 

3M Infection Prevention Division 

3M Canada Company

 

P.O. Box 5757 

London, Ontario N6A 4T1 Canada 

1 800 563-2921

3M Center

,

 

Building 275-4W-02 

St. Paul, MN 55144-1000 USA 

1 800 228-3957 

3M.com/HealthCare

3M, 3M Science. Applied to Life. and Attest 

are trademarks of 3M. Used under license in 

Canada. Please recycle. Printed in Canada. 

© 2017, 3M. All rights reserved. 1708-09713-E

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