Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Using the In
‑
line Filter 200 Micron Accessory Set
Caution: When you attach a bag to the cell expansion set, ensure that the bag is attached to the proper line and
that the sterile weld is completely open before starting any tasks.
Caution:
When using the In
‑
line Filter 200 Micron Accessory Set, sterile weld to the blue luer end first or you may
lose the cell product.
1. Use the sterile tubing welder to attach the in
‑
line filter line that has a blue luer to the cell line of the
cell expansion set.
Note:
The in
‑
line filter has two lines coming out of the top that are meant for attaching the cell inlet bag. These
lines are each a different size and are made of different materials to accommodate various sizes and types of
tubing. Choose the appropriate tubing size and type before you attach the cell product, or a sterile weld failure
could occur.
2. Use the sterile tubing welder to attach the in-line filter line to the cell inlet bag that contains the inlet
fluid that needs to be filtered.
3. Hang the bag on the bag pole.
4. Prime the in-line filter by squeezing it until the bottom of the filter contains fluid, as shown in
Figure 5-1. Figure 5-2 shows the in-line filter in use.
Note: You do not need to fill the entire filter with fluid for it to function properly.
Figure 5-1: Priming the in-line filter
Operator Procedures
5-4 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1