Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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IC EC Washout Through Membrane
The purpose of this task is to replace small molecule components on the IC side that pass through the
membrane either by diffusion or by ultrafiltration. IC components retained by the membrane are not
removed from the IC circulation loop. Components on the EC side are washed out by fluid replacement.
The replacement volume is specified by the number of IC Volumes and EC Volumes exchanged.
Table 9-7 describes the types of solution that are needed to attach to each line when performing IC EC
Washout Through Membrane. These solutions and corresponding volumes are based on the factory
default settings for this task.
Table 9-7: Solutions for IC EC Washout Through Membrane
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell None N/A
Reagent None N/A
IC Media Media with protein 0.3 L
Wash None N/A
EC Media Media without
protein 0.4 L
IC EC Washout Through Membrane pathway:
Task
>
Washout
>
IC EC Washout Through Membrane
1. Confirm the settings for IC EC Washout Through Membrane shown in Table 9-8.
Table 9-8: Settings for IC EC Washout Through Membrane
Setting Factory Default Setting Options
IC Inlet IC Media Reagent, IC Media, Wash, EC Media
IC Inlet Rate 100 mL/min N/A
The system automatically calculates this rate based on the EC Inlet Rate
and the number of IC Volumes and EC Volumes used for the Exchange
stop condition for this task.
IC Circulation Rate −59 mL/min N/A
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet EC Media Reagent, IC Media, Wash, EC Media
IC EC Washout Through Membrane
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 9-7