Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 131 / 204

Load Cells With Circulation
The purpose of this task is to load the cells into the bioreactor from the cell inlet bag until the bag is
empty and to use IC circulation to distribute the cells. This task includes three separate steps:
•
Step 1: loads the cells into the bioreactor.
Note: One method for determining the volume in the cell inlet bag (step 1) is to use a volume that is an integer
multiple of the IC Inlet Rate.
• Step 2: chases the cells from the ARC into the IC circulation loop.
• Step 3: chases the cells from the IC circulation loop into the bioreactor.
Table 10-1 describes the types of solution that are needed to attach to each line when performing Load
Cells With Circulation. These solutions and corresponding volumes are based on the factory default
settings for this task.
As a precondition before starting this task:
Fill the cell inlet bag with a minimum of approximately 40 mL of air.
Table 10-1: Solutions for Load Cells With Circulation
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell Cells As desired
Reagent None N/A
IC Media Media with protein 0.2 L
Wash None N/A
EC Media None N/A
Load Cells With Circulation pathway:
Task
>
Load and Attach
>
Load Cells With Circulation
1.
Confirm the settings for step 1 shown in Table 10-2.
Table 10-2: Step 1 Settings for Load Cells With Circulation
Setting Factory Default Setting Options
IC Inlet Cell N/A
IC Inlet Rate 50 mL/min 20 to 100 mL/min
IC Circulation Rate 139 mL/min 20 to 300 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this step.
EC Inlet None N/A
Load and Attach Tasks
10-2 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1