Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 134 / 204

Load Cells Without Circulation
The purpose of this task is to load the cells into the bioreactor from the cell inlet bag until the bag is
empty. This task does not use IC circulation to distribute the cells. This task includes two separate steps:
•
Step 1: loads the cells from the cell inlet bag into the bioreactor.
• Step 2: chases the cells from the ARC to the bioreactor. Larger chase volumes spread the cells and
move them towards the IC outlet.
Table 10-5 describes the types of solution that are needed to attach to each line when performing Load
Cells Without Circulation. These solutions and corresponding volumes are based on the factory default
settings for this task.
As preconditions before starting this task:
•
Fill the cell inlet bag with a minimum of approximately 40 mL of air.
• Follow the instructions in “Using the In
‑
Line Filter 200 Micron Accessory Set” on page 5
‑
4 to add
the in-line filter to the cell line of the cell expansion set, if desired.
Table 10-5: Solutions for Load Cells Without Circulation
Line
Solution Attached to the
Line
Required Volume (estimate based on factory
default settings)
Cell Cells As desired
Reagent None N/A
IC Media Media with protein 0.1 L
Wash None N/A
EC Media None N/A
Load Cells Without Circulation pathway:
Task
>
Load and Attach
>
Load Cells Without Circulation
1. Confirm the settings for step 1 shown in Table 10-6.
Table 10-6: Step 1 Settings for Load Cells Without Circulation
Setting Factory Default Setting Options
IC Inlet Cell N/A
IC Inlet Rate 50 mL/min 20 to 100 mL/min
IC Circulation Rate 0 mL/min N/A
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
EC Circulation Rate 30 mL/min 10 to 300 mL/min
Load Cells Without Circulation
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 10-5