Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Table 12-8: Step 2 Settings for Release Adherent Cells And Harvest (continued)
Setting Factory Default Setting Options
EC Circulation Rate 30 mL/min 0 to 300 mL/min
Outlet EC Outlet N/A
Rocker In Motion (−90°, 180°, 1 sec) In Motion (−180° to 270°, 0 to 3600 sec)
Stop Condition Empty Bag N/A
3. Confirm the settings for step 3 shown in Table 12-9.
Table 12-9: Step 3 Settings for Release Adherent Cells And Harvest
Setting Factory Default Setting Options
IC Inlet Wash IC Media, Wash, EC Media
IC Inlet Rate 50 mL/min Same as step 2
IC Circulation Rate 300 mL/min Same as step 2
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
EC Circulation Rate 30 mL/min Same as step 2
Outlet EC Outlet N/A
Rocker In Motion (−90°, 180°, 1 sec) Same as step 2
Stop Condition IC Volume (22 mL) IC Volume (1 to 100 mL)
4. Confirm the settings for step 4 shown in Table 12-10.
Table 12-10: Step 4 Settings for Release Adherent Cells And Harvest
Setting Factory Default Setting Options
IC Inlet None N/A
IC Inlet Rate 0 mL/min N/A
IC Circulation Rate 300 mL/min 30 to 300 mL/min
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
EC Circulation Rate 30 mL/min 0 to 300 mL/min
Release Adherent Cells And Harvest
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Cell Expansion System • Operator's Manual for Software Version 2.1 12-7