Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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High Density Washout
The purpose of this task is to remove any non
‑
adhered cells from the bioreactor after loading 25 mL to
62 mL of bone marrow. The types of cells removed include red blood cells, platelets, and non-adherent
mononuclear cells.
This task is also a useful washout task for any occasion when the bioreactor is packed with a large number
of cells. When loading 10 mL or less of bone marrow, the Rapid IC Washout task is recommended.
When loading between 10 mL and 25 mL of bone marrow, no specific guidelines exist; using this task in
such cases should not cause problems but may not be necessary.
Table 9-1 describes the types of solution that are needed to attach to each line when performing High
Density Washout. These solutions and corresponding volumes are based on the factory default settings
for this task.
Table 9-1: Solutions for High Density Washout
Line
Solution Attached to the
Line
Required Volume (estimate based on factory
default settings)
Cell None N/A
Reagent None N/A
IC Media Media with protein Approximately 0.5 L
Wash None N/A
EC Media Media without protein Approximately 0.5 L
High Density Washout pathway:
Task
>
Washout
>
High Density Washout
1. Confirm the settings for High Density Washout shown in Table 9-2.
Table 9-2: Settings for High Density Washout
Setting Factory Default Setting Options
IC Inlet IC Media IC Media, Wash, EC Media
Maximum IC Inlet
Rate 100 mL/min 80 to 200 mL/min
EC Inlet EC Media IC Media, Wash, EC Media
EC Circulation Rate 30 mL/min 10 to 300 mL/min
Stop Condition Inlet Volume (1000 mL) Inlet Volume (400 to 4000 mL)
2.
Touch
Start
.
3. Check the status line to confirm that the task has started.
When the system completes the task, the home screen appears, the system changes to an idle state,
and the status line displays Idle.
Washout Tasks
9-2 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1