Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 124 / 204

IC EC Washout
The purpose of this task is to replace the fluid in both the IC circulation loop and the EC circulation
loop. The replacement volume is specified by the number of IC Volumes and EC Volumes exchanged.
Table 9-5 describes the types of solution that are needed to attach to each line when performing IC EC
Washout. These solutions and corresponding volumes are based on the factory default settings for this
task.
Table 9-5: Solutions for IC EC Washout
Line
Solution Attached to the
Line
Required Volume (estimate based on factory
default settings)
Cell None N/A
Reagent None N/A
IC Media Media with protein 0.6 L
Wash None N/A
EC Media Media without protein 0.9 L
IC EC Washout pathway:
Task
>
Washout
>
IC EC Washout
1. Confirm the settings for IC EC Washout shown in Table 9-6.
Table 9-6: Settings for IC EC Washout
Setting Factory Default Setting Options
IC Inlet IC Media Reagent, IC Media, Wash, EC Media
IC Inlet Rate 100 mL/min N/A
The system automatically calculates this rate based on the EC Inlet Rate
and the number of IC Volumes and EC Volumes used for the Exchange
stop condition for this task.
IC Circulation Rate −17 mL/min N/A
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet EC Media Reagent, IC Media, Wash, EC Media
EC Inlet Rate 148 mL/min N/A
The system automatically calculates this rate based on the number of
IC Volumes and EC Volumes used for the Exchange stop condition for this
task.
IC EC Washout
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 9-5