Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 163 / 204

Table 12-10: Step 4 Settings for Release Adherent Cells And Harvest (continued)
Setting Factory Default Setting Options
Outlet EC Outlet N/A
Rocker In Motion (−90°, 180°, 1 sec) Same as step 2
Stop Condition Time (4.0 min) Time (0.1 to 20 min)
5. Confirm the settings for step 5 shown in Table 12-11.
Table 12-11: Step 5 Settings for Release Adherent Cells And Harvest
Setting Factory Default Setting Options
IC Inlet IC Media Reagent, IC Media, Wash, EC Media
IC Inlet Rate 400 mL/min 100 to 500 mL/min
IC Circulation Rate −70 mL/min −87 to −18 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this step.
EC Inlet EC Media Reagent, IC Media, Wash, EC Media
EC Inlet Rate 60 mL/min 0 to 100 mL/min
EC Circulation Rate 30 mL/min 0 to 300 mL/min
Outlet Harvest N/A
Rocker In Motion (−90°, 180°, 1 sec) In Motion (−180° to 270°, 0 to 3600 sec)
Stop Condition IC Volume (378 mL) IC Volume (50 to 1000 mL)
6.
Touch
Start
.
7. Check the status line to confirm that the task has started.
When the system completes the task, the home screen appears, the system changes to an idle state,
and the status line displays Idle.
8.
Seal and remove the harvest bag.
Release and Harvest Tasks
12-8 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1