Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 161 / 204

1. Confirm the settings for step 1 shown in Table 12-7.
Table 12-7: Step 1 Settings for Release Adherent Cells And Harvest
Setting Factory Default Setting Options
IC Inlet Wash Reagent, IC Media, Wash, EC Media
IC Inlet Rate 100 mL/min N/A
The system automatically calculates this rate based on the EC Inlet Rate
and the number of IC Volumes and EC Volumes used for the Exchange
stop condition for this step.
IC Circulation Rate −17 mL/min N/A
The system automatically calculates this rate based on the IC Inlet Rate
for this step.
EC Inlet Wash Reagent, IC Media, Wash, EC Media
EC Inlet Rate 148 mL/min N/A
The system automatically calculates this rate based on the number of
IC Volumes and EC Volumes used for the Exchange stop condition for this
step.
EC Circulation Rate −1.7 mL/min N/A
The system automatically calculates this rate based on the EC Inlet Rate
for this step.
Outlet IC and EC Outlet EC Outlet, IC Outlet, IC and EC Outlet
Rocker In Motion (−90°, 180°, 1 sec) In Motion (−180° to 270°, 0 to 3600 sec), Stationary
(−180° to 270°)
Stop Condition Exchange (2.5 IC Volumes)
(2.5 EC Volumes) Exchange (0.5 to 5.0 IC Volumes)
(0.5 to 5.0 EC Volumes)
2.
Confirm the settings for step 2 shown in Table 12-8.
Table 12-8: Step 2 Settings for Release Adherent Cells And Harvest
Setting Factory Default Setting Options
IC Inlet Reagent Cell, Reagent
IC Inlet Rate 50 mL/min 20 to 100 mL/min
IC Circulation Rate 300 mL/min 30 to 300 mL/min
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
Release and Harvest Tasks
12-6 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1