Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Coat Bioreactor
The purpose of this task is to coat the bioreactor membrane with a reagent.
•
Step 1: loads a reagent into the IC circulation loop until the bag is empty.
• Step 2: chases the reagent from the ARC into the IC circulation loop.
• Step 3: circulates the reagent in the IC circulation loop.
Table 8-4 describes the types of solution that are needed to attach to each line when performing Coat
Bioreactor. These solutions and corresponding volumes are based on the factory default settings for this
task.
As a precondition before starting this task:
Fill the cell inlet bag with a minimum of approximately 40 mL of air.
Table 8-4: Solutions for Coat Bioreactor
Line
Solution Attached to the
Line
Required Volume (estimate based on factory
default settings)
Cell None N/A
Reagent Reagent As desired
IC Media None N/A
Wash Wash solution 0.1 L plus 6 mL/hr
EC Media None N/A
Coat Bioreactor pathway:
Task
>
System Management
>
Coat Bioreactor
1. Confirm the settings for step 1 shown in Table 8-5.
Table 8-5: Step 1 Settings for Coat Bioreactor
Setting Factory Default Setting Options
IC Inlet Reagent Cell, Reagent
IC Inlet Rate 10 mL/min 0.1 to 100 mL/min
IC Circulation Rate 100 mL/min −300 to 300 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this step.
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
EC Circulation Rate 30 mL/min 0 to 100 mL/min
System Management Tasks
8-8 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1