Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 67 / 204

Preparing the System
This section contains instructions for filling the media bags and the cell inlet bags. You should have the
following supplies:
•
A media bag
–
Fluid
– A tubing pump
• A cell inlet bag
–
Fluid
– A syringe or a similar device that has a male luer connector
• A tubing sealer
Filling the Media Bags
Complete the steps in this section to fill the media bags.
1. Open the pouch that contains the media bag, and remove the contents.
2. Remove the protective cover from the end of the tubing.
3. Connect the end of the tubing to the fluid source; for example, place the tubing into the fluid
container. Use an appropriate method to avoid contamination of the fluid.
4. Load the white tube into the tubing pump.
5. Ensure that the cap on the pressure relief valve on the filter is tight.
6. Set the tubing pump to a low flow rate, such as 150 mL/min, to prime the filter. When priming the
filter, hold it upright.
After the filter cartridge is full and the fluid begins to flow into the bag, you can slowly increase the
flow rate of the tubing pump.
Note: Ensure that the opening to the white tube remains below the level of the fluid in the fluid source.
7. When the last of the fluid has been pumped from the fluid source:
a. Set the tubing pump to a low flow rate.
b. Slowly pump the fluid from the white tubing into the inlet of the filter.
c. Stop the pump.
Note: This filter has not been validated for maintaining sterility if air is forced through the membrane.
8. Use a tubing sealer to double
‑
seal the clear tubing. To ensure that you will have enough tubing to
attach the bag to the cell expansion set using a sterile tubing welder, leave as much of the clear tubing
as possible connected to the bag.
9. Separate the media bag from the filter at the double seal.
Operator Procedures
5-2 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1