Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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3. Confirm the settings for step 3 shown in Table 12-4.
Table 12-4: Step 3 Settings for Release Adherent Cells
Setting Factory Default Setting Options
IC Inlet Wash IC Media, Wash, EC Media
IC Inlet Rate 50 mL/min Same as step 2
IC Circulation Rate 300 mL/min Same as step 2
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
EC Circulation Rate 30 mL/min Same as step 2
Outlet EC Outlet N/A
Rocker In Motion (−90°, 180°, 1 sec) Same as step 2
Stop Condition IC Volume (22 mL) IC Volume (1 to 100 mL)
4.
Confirm the settings for step 4 shown in Table 12-5.
Table 12-5: Step 4 Settings for Release Adherent Cells
Setting Factory Default Setting Options
IC Inlet None N/A
IC Inlet Rate 0 mL/min N/A
IC Circulation Rate 300 mL/min 30 to 300 mL/min
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
EC Circulation Rate 30 mL/min 0 to 300 mL/min
Outlet EC Outlet N/A
Rocker In Motion (−90°, 180°, 1 sec) Same as step 2
Stop Condition Time (4.0 min) Time (0.1 to 20 min)
5. Touch
Start
.
6. Check the status line to confirm that the task has started.
7. Verify that there are no contents left in the bag.
When the system completes the task, the home screen appears, the system changes to an idle state,
and the status line displays Idle.
Release and Harvest Tasks
12-4 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1