Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 105 / 204

Prime Cell Expansion Set
The purpose of this task is to remove air from the set. Before you begin this task, you should have a media
bag available that contains at least 2 L of desired electrolyte solution, such as PBS (phosphate buffered
saline) or HBSS (Hank's Buffered Salt Solution), to use for priming the cell expansion set.
Caution: When priming the cell expansion set, do not connect the fluids to the cell expansion set before you are
instructed to do so.
Caution:
Do not use chilled fluid to prime the cell expansion set.
1. Turn on the external gas supply.
2. Attach the media bag to the cell line, and hang the bag on the bag pole.
3. Touch
Task
.
4. Touch
Set Management
.
5. Touch
Prime Cell Expansion Set
.
The Setup: Prime Cell Expansion Set screen appears.
6. Touch
Start
.
Priming begins, and the system updates the Prime Cell Expansion Set status screen and the status
message at each step throughout the prime process.
Note: During the final step of prime, the bioreactor is oriented at 180°.
7.
Take a sample from the sample port to prime the filter. See “Taking a Sample from the Sample
Port” on page 5-7 for sampling instructions.
8. Touch
Finish
when you are finished sampling.
9.
Touch
Yes
.
The home screen appears, the system changes to an idle state, the status line displays Idle, and the
bioreactor moves to the home position of 0°.
10. Seal and remove the waste bag on the outlet line. Then use a sterile tubing welder to attach a
replacement bag to the outlet line, and hang the bag on the bag pole.
11.
Separate the tubing lines, as described in “Separating the Tubing Lines” on page 7-11.
Set Management Tasks
7-10 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1