Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 106 / 204

Separating the Tubing Lines
Note: Each line has two white tags that state the name of that line.
After the system primes the cell expansion set, you must manually separate the lines of the EC media/
reagent loop and the IC media/wash loop, as outlined in the following instructions. Before you begin,
remove the tape from the coils of lines if you have not already done so.
Caution: When you seal each line loop, seal it in a location that ensures that each resulting line has a name tag. For
example, the EC media tag should be located on one line and the reagent tag on the other line; the IC media tag should
be located on one line and the wash tag on the other line.
Note:
All seals should be double seals.
1.
Find the midpoint between the EC media tag and the reagent tag, and seal the line at that point with
a tubing sealer.
2. Find the midpoint between the IC media tag and the wash tag, and seal the line at that point with a
tubing sealer.
3.
Separate each loop at the seal to divide each loop into two lines.
Prime Cell Expansion Set
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 7-11